Hotspot

rps18

LSC · 70667-70972 · 306 bp

Back to Bergenia

Pi 0.00436

MarkerSeek score 30.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 2
pi: 0.00436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGGATTTTCAGGGGTAAGGA	TCTGCGTTTGAGTTCGGATT	45.0	45.0	55.4	57.8	385 bp	1.0	58.2
rps18_p2	AGGATTTTCAGGGGTAAGGA	CTGCGTTTGAGTTCGGATTG	45.0	50.0	55.4	57.8	384 bp	1.0	57.7
rps18_p3	AGGATTTTCAGGGGTAAGGAA	TCTGCGTTTGAGTTCGGATT	42.9	45.0	56.2	57.8	385 bp	1.0	56.8
rps18_p4	AGGATTTTCAGGGGTAAGGA	ATCTGCGTTTGAGTTCGGAT	45.0	45.0	55.4	57.6	386 bp	1.0	56.4
rps18_p5	AGGATTTTCAGGGGTAAGGAA	CTGCGTTTGAGTTCGGATTG	42.9	50.0	56.2	57.8	384 bp	1.0	56.3