Hotspot

ndhF

SSC · 112324-114558 · 2235 bp

Back to Bergenia

Pi 0.00626

MarkerSeek score 46.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 21
pi: 0.00626
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAACCTCTTGTGACCCGTC	ACGGCGATATCATTTGACCA	50.0	45.0	58.0	57.4	2517-2628 bp	1.0	76.2
ndhF_p2	AAGAGGGATCCACCGAAGAA	ACGGCGATATCATTTGACCA	50.0	45.0	58.0	57.4	2776-2887 bp	1.0	76.0
ndhF_p3	GGGAAAGCGAGGAAGAAACA	ACGGCGATATCATTTGACCA	50.0	45.0	58.1	57.4	2845-2956 bp	1.0	75.5
ndhF_p4	CTTGTGACCCGTCTTTTCGA	ACGGCGATATCATTTGACCA	50.0	45.0	58.1	57.4	2510-2621 bp	1.0	75.4
ndhF_p5	GCAGAATACCGTCACCCATT	ACGGCGATATCATTTGACCA	50.0	45.0	58.0	57.4	2908-2916 bp	0.666667	62.7