Hotspot

trnV-GAC-rps12

IRa · 140508-142026 · 1519 bp

Back to Bergenia

Pi 0.00219
MarkerSeek score 60.10
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1519
variable_sites
5
pi
0.00219
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 CGGGGGAGCAAGTCAAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1676-1691 bp 1.0 77.2
trnV-GAC-rps12_p2 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1615-1630 bp 1.0 76.9
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1619-1634 bp 1.0 76.8
trnV-GAC-rps12_p4 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1614-1629 bp 1.0 76.8
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1617-1632 bp 1.0 75.9