Hotspot

ycf1-ndhF

SSC · 112286-112323 · 38 bp

Back to Bergenia

Pi 0.193

MarkerSeek score 72.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 38
variable_sites: 11
pi: 0.193
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAAACCTCTTGTGACCCGTC	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.0	57.8	179-291 bp	1.0	84.7
ycf1-ndhF_p2	CTTGTGACCCGTCTTTTCGA	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.1	57.8	172-284 bp	1.0	84.1
ycf1-ndhF_p3	AAGAGGGATCCACCGAAGAA	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.0	57.8	438-550 bp	1.0	81.0
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.0	57.8	570-682 bp	1.0	80.5
ycf1-ndhF_p5	GGGAAAGCGAGGAAGAAACA	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.1	57.8	507-619 bp	1.0	80.2