Hotspot

rps12-trnV-GAC

IRb · 100720-102238 · 1519 bp

Back to Bergenia

Pi 0.00219

MarkerSeek score 60.10

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1519
variable_sites: 5
pi: 0.00219
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	45.0	50.0	57.7	57.9	1676-1691 bp	1.0	77.2
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	45.0	50.0	57.7	57.8	1615-1630 bp	1.0	76.9
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	45.0	50.0	57.7	57.8	1619-1634 bp	1.0	76.8
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	45.0	50.0	57.7	57.8	1614-1629 bp	1.0	76.8
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	45.0	55.0	57.7	58.3	1617-1632 bp	1.0	75.9