Hotspot

petA-psbJ

LSC · 65880-66602 · 723 bp

Back to Bergenia

Pi 0.0231
MarkerSeek score 75.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
723
variable_sites
25
pi
0.0231
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC ACTGGTATTCCTGTGATCGG 50.0 50.0 58.0 56.7 916-923 bp 1.0 72.9
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CTGGTATTCCTGTGATCGGT 50.0 50.0 58.0 56.7 915-922 bp 1.0 72.9
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC ACTGGTATTCCTGTGATCGG 50.0 50.0 57.8 56.7 899-906 bp 1.0 72.1
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC CTGGTATTCCTGTGATCGGT 50.0 50.0 57.8 56.7 898-905 bp 1.0 72.1
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT CTGGTATTCCTGTGATCGGT 50.0 50.0 58.2 56.7 912-919 bp 1.0 71.8