Hotspot

trnT-UGU-trnL-UAA

LSC · 48743-49754 · 1012 bp

Back to Bergenia

Pi 0.00833

MarkerSeek score 67.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 12
pi: 0.00833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	1028-1188 bp	1.0	82.6
trnT-UGU-trnL-UAA_p2	AGAGAAGCCAGCTATCGGAA	CCGTAGCGTCTACCAATTCC	50.0	55.0	58.2	58.2	1076-1236 bp	1.0	81.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1031-1191 bp	1.0	81.9
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	1060-1220 bp	1.0	81.7
trnT-UGU-trnL-UAA_p5	AGAGAAGCCAGCTATCGGAA	AGTCCGTAGCGTCTACCAAT	50.0	50.0	58.2	58.2	1079-1239 bp	1.0	81.2