Hotspot

ycf3-trnS-GGA

LSC · 46492-47361 · 870 bp

Back to Bergenia

Pi 0.00843

MarkerSeek score 66.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 9
pi: 0.00843
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	768-971 bp	1.0	75.7
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	739-942 bp	1.0	75.0
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	769-972 bp	1.0	73.4
ycf3-trnS-GGA_p4	ATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	767-970 bp	1.0	72.7
ycf3-trnS-GGA_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	766-969 bp	1.0	72.7