Hotspot

atpH-atpI

LSC · 14371-15435 · 1065 bp

Back to Bergenia

Pi 0.00689

MarkerSeek score 63.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1065
variable_sites: 11
pi: 0.00689
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCAGCGG	45.0	45.0	58.0	57.9	1152-1244 bp	1.0	79.8
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCAGCGG	45.0	45.0	57.9	57.9	1144-1236 bp	1.0	79.6
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCAGCGG	50.0	45.0	57.9	57.9	1208-1300 bp	1.0	79.1
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCAGCGG	45.0	45.0	58.2	57.9	1145-1237 bp	1.0	78.8
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCAGCGG	45.0	45.0	58.2	57.9	1153-1245 bp	1.0	78.6