Hotspot

rps16-trnQ-UUG

LSC · 6077-7529 · 1453 bp

Back to Bergenia

Pi 0.0117

MarkerSeek score 63.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1453
variable_sites: 21
pi: 0.0117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1028-1727 bp	1.0	83.5
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1039-1738 bp	1.0	82.9
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1049-1748 bp	1.0	81.2
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1050-1749 bp	1.0	81.2
rps16-trnQ-UUG_p5	ATCCACAGCTGATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	1051-1750 bp	1.0	79.5