Hotspot

rps15-trnN-GUU

SSC · 128917-135295 · 6379 bp

Back to Berchemiella

Pi 0.00219
MarkerSeek score 46.30
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6379
variable_sites
21
pi
0.00219
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CAGGCCTCAATATCGACCTT CGAAGAACCCGTCTATTTATGGA 50.0 43.5 57.1 58.1 232 bp 1.0 49.2
rps15-trnN-GUU_p2 CCAGGCCTCAATATCGACCT CGAAGAACCCGTCTATTTATGGA 55.0 43.5 58.9 58.1 233 bp 1.0 49.0
rps15-trnN-GUU_p3 TCCAGGCCTCAATATCGACC CGAAGAACCCGTCTATTTATGGA 55.0 43.5 59.0 58.1 234 bp 1.0 48.9
rps15-trnN-GUU_p4 GATCCAGGCCTCAATATCGAC CGAAGAACCCGTCTATTTATGGA 52.4 43.5 58.0 58.1 236 bp 1.0 48.3
rps15-trnN-GUU_p5 CAGGCCTCAATATCGACCTT TGAATATACGAAGAACCCGTCT 50.0 40.9 57.1 56.7 240 bp 1.0 47.6