Hotspot

rpl32-trnL-UAG

SSC · 117983-119443 · 1461 bp

Back to Berchemiella

Pi 0.0069
MarkerSeek score 63.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1461
variable_sites
15
pi
0.0069
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGAAGGGATATTGGGTAGCA AGCGTGTCTACCGATTTCAC 47.6 50.0 57.9 58.0 1574-1586 bp 1.0 72.7
rpl32-trnL-UAG_p2 AGGAAGGGATATTGGGTAGCA CAGCGTGTCTACCGATTTCA 47.6 50.0 57.9 58.0 1575-1587 bp 1.0 72.7
rpl32-trnL-UAG_p3 AGGAAGGGATATTGGGTAGCA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 1584-1596 bp 1.0 71.8
rpl32-trnL-UAG_p4 GCGAAGTCTCTTTCTACGAGG AGCGTGTCTACCGATTTCAC 52.4 50.0 58.2 58.0 1535-1547 bp 1.0 71.6
rpl32-trnL-UAG_p5 GCGAAGTCTCTTTCTACGAGG CAGCGTGTCTACCGATTTCA 52.4 50.0 58.2 58.0 1536-1548 bp 1.0 71.6