Hotspot

petD-rpoA

LSC · 82181-82389 · 209 bp

Back to Berchemiella

Pi 0.0191

MarkerSeek score 65.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 6
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	AGCCCTTTGGTTGGGTATTG	AGAAATAGAAAAGCACTTCGCA	50.0	36.4	58.1	56.6	301-302 bp	1.0	54.7
petD-rpoA_p2	CAGCCCTTTGGTTGGGTATT	AGAAATAGAAAAGCACTTCGCA	50.0	36.4	58.1	56.6	302-303 bp	1.0	54.7
petD-rpoA_p3	CAGTAGCGACAACCGTCTTT	AGAAATAGAAAAGCACTTCGCA	50.0	36.4	58.2	56.6	338-339 bp	1.0	53.2
petD-rpoA_p4	TTGGGTATTGGGGCAACATT	AGAAATAGAAAAGCACTTCGCA	45.0	36.4	57.7	56.6	291-292 bp	1.0	52.6
petD-rpoA_p5	AGCGACAACCGTCTTTTTGA	AGAAATAGAAAAGCACTTCGCA	45.0	36.4	58.3	56.6	334-335 bp	1.0	52.3