Hotspot

rpl20-rps12

LSC · 73200-74017 · 818 bp

Back to Berchemiella

Pi 0.00326

MarkerSeek score 61.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 4
pi: 0.00326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AGAAAGTCGTGAATGAGCCC	CTCAACGACGAGGAACATGT	50.0	50.0	57.9	57.9	946-950 bp	1.0	79.2
rpl20-rps12_p2	AGAAAGTCGTGAATGAGCCC	AATGTCACGAAATCCCCTGC	50.0	50.0	57.9	58.5	980-984 bp	1.0	75.4
rpl20-rps12_p3	GTGAATGAGCCCCTCGAAAG	CTCAACGACGAGGAACATGT	55.0	50.0	58.6	57.9	938-942 bp	1.0	75.0
rpl20-rps12_p4	CCCTCGAAAGCTTGATGCAA	CTCAACGACGAGGAACATGT	50.0	50.0	58.8	57.9	928-932 bp	1.0	73.3
rpl20-rps12_p5	GAAAGTCGTGAATGAGCCCC	CTCAACGACGAGGAACATGT	55.0	50.0	58.9	57.9	945-949 bp	1.0	72.7