Hotspot

psaJ-rpl33

LSC · 71368-71821 · 454 bp

Back to Berchemiella

Pi 0.0117

MarkerSeek score 63.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 8
pi: 0.0117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGTTTATTTCCGGATGCGT	TCTGGAAATGCCCGTTGATT	45.0	45.0	58.0	57.8	596-599 bp	1.0	79.3
psaJ-rpl33_p2	TCGTTTATTTCCGGATGCGT	TGGAAATGCCCGTTGATTCT	45.0	45.0	58.0	57.8	594-597 bp	1.0	79.3
psaJ-rpl33_p3	TCGTTTATTTCCGGATGCGT	TTTCGAACACAACCGGTACA	45.0	45.0	58.0	57.7	562-565 bp	1.0	78.6
psaJ-rpl33_p4	TCGTTTATTTCCGGATGCGT	GGACATCTTTACCCTTCGCC	45.0	55.0	58.0	58.3	522-525 bp	1.0	78.4
psaJ-rpl33_p5	TGGTTCGGTTCTTTAGCAGG	TGGAAATGCCCGTTGATTCT	50.0	45.0	57.8	57.8	632-635 bp	1.0	77.9