Hotspot

psbE-petL

LSC · 68641-70003 · 1363 bp

Back to Berchemiella

Pi 0.00318

MarkerSeek score 61.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1363
variable_sites: 6
pi: 0.00318
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCCGATAAATAGAGCCGAG	50.0	55.0	57.9	58.0	1537-1540 bp	1.0	80.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GCCCGATAAATAGAGCCGAG	50.0	55.0	57.8	58.0	1460-1463 bp	1.0	79.4
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GCCCGATAAATAGAGCCGAG	45.0	55.0	58.2	58.0	1546-1549 bp	1.0	79.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	GCCCGATAAATAGAGCCGAG	50.0	55.0	58.2	58.0	1551-1554 bp	1.0	79.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	GCCCGATAAATAGAGCCGAG	50.0	55.0	57.8	58.0	1545-1548 bp	1.0	79.2