Hotspot

accD-psaI

LSC · 62906-63665 · 760 bp

Back to Berchemiella

Pi 0.00527

MarkerSeek score 62.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 6
pi: 0.00527
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTTTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	917-918 bp	1.0	80.0
accD-psaI_p2	AAGGGCGTTTTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	916-917 bp	1.0	80.0
accD-psaI_p3	AAAGGGCGTTTTGAGTGAGT	AGAAACCATTGCAATTGCCG	45.0	45.0	57.9	57.9	922-923 bp	1.0	79.5
accD-psaI_p4	AAGGGCGTTTTGAGTGAGTT	AGAAACCATTGCAATTGCCG	45.0	45.0	57.9	57.9	921-922 bp	1.0	79.5
accD-psaI_p5	AGGGCGTTTTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.1	58.0	915-916 bp	1.0	73.0