Hotspot

rbcL-accD

LSC · 60548-61378 · 831 bp

Back to Berchemiella

Pi 0.000808

MarkerSeek score 60.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 1
pi: 0.000808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCAGCTTGTGAAGTATG	AACATCGAATCGAACCACCT	50.0	45.0	57.5	57.2	917-923 bp	1.0	70.5
rbcL-accD_p2	GCTGCAGCTTGTGAAGTATG	ACATCGAATCGAACCACCTT	50.0	45.0	57.5	57.2	916-922 bp	1.0	70.5
rbcL-accD_p3	GCTGCAGCTTGTGAAGTATG	CAACATCGAATCGAACCACC	50.0	50.0	57.5	57.2	918-924 bp	1.0	70.1
rbcL-accD_p4	GCTGCAGCTTGTGAAGTATG	TCCTCACTTTCACTGGCATT	50.0	45.0	57.5	57.0	1007-1013 bp	1.0	68.8
rbcL-accD_p5	GCTGCAGCTTGTGAAGTATG	ACATCGAATCGAACCACCTTT	50.0	42.9	57.5	57.9	916-922 bp	1.0	68.1