Hotspot

atpB-rbcL

LSC · 58292-59119 · 828 bp

Back to Berchemiella

Pi 0.00323

MarkerSeek score 61.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 4
pi: 0.00323
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATACGCCCCAGGTTGTTTT	GCCTTGAATCCAACACTTGC	45.0	50.0	58.0	57.9	939-941 bp	1.0	79.9
atpB-rbcL_p2	ATACGCCCCAGGTTGTTTTT	GCCTTGAATCCAACACTTGC	45.0	50.0	58.0	57.9	938-940 bp	1.0	79.9
atpB-rbcL_p3	AATACGCCCCAGGTTGTTTT	CCAGCCTTGAATCCAACACT	45.0	50.0	58.0	58.1	942-944 bp	1.0	79.8
atpB-rbcL_p4	ATACGCCCCAGGTTGTTTTT	CCAGCCTTGAATCCAACACT	45.0	50.0	58.0	58.1	941-943 bp	1.0	79.8
atpB-rbcL_p5	GCTACATCCAATACCGGACC	GCCTTGAATCCAACACTTGC	55.0	50.0	57.8	57.9	971-973 bp	1.0	78.6