Pi
0.00485
MarkerSeek score
58.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AACAGATGGAGAAAAGCCGG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 1268-1289 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p2 | AACAGATGGAGAAAAGCCGG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.2 | 58.1 | 1276-1297 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1243-1264 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p4 | ATGGAGAAAAGCCGGCTATC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 1263-1284 bp | 1.0 | 79.0 |
| trnT-UGU-trnL-UAA_p5 | GATGGAGAAAAGCCGGCTAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 1264-1285 bp | 1.0 | 79.0 |