Hotspot

psaA-ycf3

LSC · 44854-45603 · 750 bp

Back to Berchemiella

Pi 0.00267

MarkerSeek score 60.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 3
pi: 0.00267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	927-928 bp	1.0	79.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	869-870 bp	1.0	78.6
psaA-ycf3_p3	CGGGGTCCCTATCTACCAAA	ATCAAGCTGCCGAGTATTGG	55.0	50.0	58.2	58.0	902-903 bp	1.0	78.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	929-930 bp	1.0	78.3
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	ATCAAGCTGCCGAGTATTGG	55.0	50.0	57.8	58.0	945-946 bp	1.0	78.2