Hotspot

psbM-trnD-GUC

LSC · 32140-32608 · 469 bp

Back to Berchemiella

Pi 0.0057

MarkerSeek score 46.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 4
pi: 0.0057
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	45.0	66.7	55.8	60.1	536-537 bp	1.0	40.7
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	GTTCGAGCCCCGTCAGTC	40.9	66.7	57.4	60.1	543-544 bp	1.0	40.7
psbM-trnD-GUC_p3	TCGCCTTGACTTACTGTTTT	GTTCGAGCCCCGTCAGTC	40.0	66.7	55.2	60.1	590-591 bp	1.0	40.7
psbM-trnD-GUC_p4	GGAACTAGAATGAACAGTGCAG	GTTCGAGCCCCGTCAGTC	45.5	66.7	57.1	60.1	542-543 bp	1.0	40.7
psbM-trnD-GUC_p5	TCGCCTTGACTTACTGTTTTT	GTTCGAGCCCCGTCAGTC	38.1	66.7	56.0	60.1	590-591 bp	1.0	40.7