Hotspot

rpoB-trnC-GCA

LSC · 28444-29680 · 1237 bp

Back to Berchemiella

Pi 0.00647

MarkerSeek score 63.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 12
pi: 0.00647
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCCGATCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1389-1394 bp	1.0	77.5
rpoB-trnC-GCA_p2	ACTCTTCTGTTAAGCCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1399-1404 bp	1.0	77.3
rpoB-trnC-GCA_p3	GCCCCGATCAATGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.4	58.1	1386-1391 bp	1.0	77.0
rpoB-trnC-GCA_p4	AACTCTTCTGTTAAGCCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1400-1405 bp	1.0	76.4
rpoB-trnC-GCA_p5	AGCCCCGATCAATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1387-1392 bp	1.0	76.3