Hotspot

trnS-GCU-trnG-UCC

LSC · 8800-9955 · 1156 bp

Back to Berchemiella

Pi 0.00849

MarkerSeek score 65.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1156
variable_sites: 14
pi: 0.00849
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TTAATCAAACCGAGGGACCC	50.0	50.0	58.0	57.2	1257-1270 bp	1.0	75.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TTAATCAAACCGAGGGACCC	50.0	50.0	58.0	57.2	1256-1269 bp	1.0	75.8
trnS-GCU-trnG-UCC_p3	CAAAGTGTCGACGAAAACGG	TTAATCAAACCGAGGGACCC	50.0	50.0	58.0	57.2	1327-1340 bp	1.0	75.7
trnS-GCU-trnG-UCC_p4	CCAAAGTGTCGACGAAAACG	TTAATCAAACCGAGGGACCC	50.0	50.0	58.0	57.2	1328-1341 bp	1.0	75.7
trnS-GCU-trnG-UCC_p5	AGTGTCGACGAAAACGGAAA	TTAATCAAACCGAGGGACCC	45.0	50.0	58.1	57.2	1324-1337 bp	1.0	75.1