Hotspot

rps16-trnQ-UUG

LSC · 6707-7418 · 712 bp

Back to Berchemiella

Pi 0.00563

MarkerSeek score 61.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 6
pi: 0.00563
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GTCGAATAGATTTGGGGCGT	45.0	50.0	58.1	58.1	840-845 bp	1.0	79.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TCGAATAGATTTGGGGCGTG	45.0	50.0	58.1	58.1	839-844 bp	1.0	79.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TGTCGAATAGATTTGGGGCG	45.0	50.0	58.1	58.1	841-846 bp	1.0	79.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	780-785 bp	1.0	79.5
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GTCGAATAGATTTGGGGCGT	55.0	50.0	58.4	58.1	854-859 bp	1.0	77.8