Hotspot

trnK-UUU-rps16

LSC · 4523-5549 · 1027 bp

Back to Berchemiella

Pi 0.00585

MarkerSeek score 62.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 9
pi: 0.00585
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1207 bp	1.0	80.1
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1206 bp	1.0	80.1
trnK-UUU-rps16_p3	AGTCGCGGTCTTACAAACTC	CTTGCAAAAAGGAGCTCAGC	50.0	50.0	57.9	57.9	1247 bp	1.0	79.7
trnK-UUU-rps16_p4	GTCGCGGTCTTACAAACTCT	CTTGCAAAAAGGAGCTCAGC	50.0	50.0	57.9	57.9	1246 bp	1.0	79.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	1123 bp	1.0	79.3