Hotspot

rps16

LSC · 5459-6602 · 1144 bp

Back to Berchemia

Pi 0.0726

MarkerSeek score 84.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1144
variable_sites: 203
pi: 0.0726
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTTGTGTTGGATTGGCACT	AGCATACCCAACCCGCTATA	45.0	50.0	57.9	58.3	2310-2323 bp	0.8	72.2
rps16_p2	GGCTAAAGGCTGTAGACGAC	AGCATACCCAACCCGCTATA	55.0	50.0	58.1	58.3	2226-2244 bp	0.8	71.8
rps16_p3	ACTTGTGTTGGATTGGCACT	CGGCCGAACCAATGACTATT	45.0	50.0	57.9	58.3	1617-1647 bp	0.8	71.7
rps16_p4	TAGAACAGGTAAGGGTGGGG	AGCATACCCAACCCGCTATA	55.0	50.0	58.0	58.3	2003-2006 bp	0.6	63.4
rps16_p5	TAGAACAGGTAAGGGTGGGG	CGGCCGAACCAATGACTATT	55.0	50.0	58.0	58.3	1319-1329 bp	0.6	62.8