Hotspot

petB

LSC · 79774-81192 · 1419 bp

Back to Berchemia

Pi 0.0542

MarkerSeek score 84.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1419
variable_sites: 187
pi: 0.0542
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGAAAGTACTGAGCCTCCCA	GTATGGGGCTTACATGGTGG	50.0	55.0	58.0	58.0	2428-2432 bp	0.8	72.4
petB_p2	TGGGAGGCTCAGTACTTCAA	GTATGGGGCTTACATGGTGG	50.0	55.0	58.0	58.0	2404-2408 bp	0.8	72.4
petB_p3	TTGGGAGGCTCAGTACTTCA	GTATGGGGCTTACATGGTGG	50.0	55.0	58.0	58.0	2405-2409 bp	0.8	72.4
petB_p4	TGGGAGGCTCAGTACTTCAA	CTCACGGAGCCTGTTCATAC	50.0	55.0	58.0	58.1	2484-2488 bp	0.8	72.0
petB_p5	TTGGGAGGCTCAGTACTTCA	CTCACGGAGCCTGTTCATAC	50.0	55.0	58.0	58.1	2485-2489 bp	0.8	72.0