Hotspot

rpl32-trnL-UAG

SSC · 118442-119862 · 1421 bp

Back to Berchemia

Pi 0.0879
MarkerSeek score 76.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1421
variable_sites
234
pi
0.0879
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.84
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGTTGGAAGGGGAATGAAA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.7 58.0 1947-2089 bp 0.8 77.5
rpl32-trnL-UAG_p2 GGGTTGGAAGGGGAATGAAA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.7 58.0 1948-2090 bp 0.8 77.5
rpl32-trnL-UAG_p3 CCCACATACGGCGAAGATTT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 2013-2155 bp 0.8 77.3
rpl32-trnL-UAG_p4 CCCACATACGGCGAAGATTT CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 2014-2156 bp 0.8 77.3
rpl32-trnL-UAG_p5 GGGTTGGAAGGGGAATGAAA CAAAAGAGCCGCATAGCCTA 50.0 50.0 57.7 58.0 2574-2716 bp 0.8 76.4