Hotspot

ndhF-rpl32

SSC · 117897-118282 · 386 bp

Back to Berchemia

Pi 0.186

MarkerSeek score 69.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 146
pi: 0.186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATATCCCCGATCATGAGCCA	GCCGAACCTTGCGTTTATTC	50.0	50.0	58.0	58.1	1343-1414 bp	0.8	77.1
ndhF-rpl32_p2	ATCCCCGATCATGAGCCATA	GCCGAACCTTGCGTTTATTC	50.0	50.0	58.0	58.1	1341-1412 bp	0.8	77.1
ndhF-rpl32_p3	TATCCCCGATCATGAGCCAT	GCCGAACCTTGCGTTTATTC	50.0	50.0	58.0	58.1	1342-1413 bp	0.8	77.1
ndhF-rpl32_p4	TCCCCGATCATGAGCCATAT	GCCGAACCTTGCGTTTATTC	50.0	50.0	58.0	58.1	1340-1411 bp	0.8	77.1
ndhF-rpl32_p5	CAAAATCCCCTACGCGGTTA	GCCGAACCTTGCGTTTATTC	50.0	50.0	58.0	58.1	1542-1613 bp	0.8	76.5