Hotspot

rpl20-rps12

LSC · 73561-74379 · 819 bp

Back to Berchemia

Pi 0.0728

MarkerSeek score 85.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 144
pi: 0.0728
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGCCTACCTCTATCCCGATG	CCTGGAAGCGGAAGAACTAC	55.0	55.0	58.1	58.0	1449-1455 bp	1.0	91.2
rpl20-rps12_p2	ATCCGAGTGATCCACAAACG	CCTGGAAGCGGAAGAACTAC	50.0	55.0	58.0	58.0	1486-1491 bp	0.8	72.7
rpl20-rps12_p3	CCACAAACGCCGAAAATCTC	CCTGGAAGCGGAAGAACTAC	50.0	55.0	58.0	58.0	1475-1480 bp	0.8	72.5
rpl20-rps12_p4	AAGCAAACGCCTCTTGTACA	CCTGGAAGCGGAAGAACTAC	45.0	55.0	58.0	58.0	1565-1570 bp	0.8	72.5
rpl20-rps12_p5	ATCCGAGTGATCCACAAACG	TCCTGGAAGCGGAAGAACTA	50.0	50.0	58.0	58.1	1487-1492 bp	0.8	72.3