Hotspot

ndhC-trnV-UAC

LSC · 54917-55472 · 556 bp

Back to Berchemia

Pi 0.146

MarkerSeek score 83.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 181
pi: 0.146
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AAACTGGAGAGTCTCGACCA	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1258-1291 bp	0.8	73.6
ndhC-trnV-UAC_p2	AACTGGAGAGTCTCGACCAA	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1257-1290 bp	0.8	73.6
ndhC-trnV-UAC_p3	CTGTGCCTGCCGTTAAAATG	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1398-1431 bp	0.8	73.4
ndhC-trnV-UAC_p4	AGAGAAGCGGCCATAAACTG	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1272-1305 bp	0.8	73.4
ndhC-trnV-UAC_p5	TCGGTTCAGGCATTTGTTCA	TGAATCAGAGCACATGGAGC	45.0	50.0	58.0	58.0	1455-1488 bp	0.8	73.3