Hotspot

psbZ-trnG-GCC

LSC · 38870-39354 · 485 bp

Back to Berchemia

Pi 0.139

MarkerSeek score 74.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 164
pi: 0.139
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	AGTGTACCCGTCGTATTTGC	CGACGTTGTTTTTCGACTGG	50.0	50.0	58.0	58.0	1230-1244 bp	0.8	73.2
psbZ-trnG-GCC_p2	GTGTACCCGTCGTATTTGCT	CGACGTTGTTTTTCGACTGG	50.0	50.0	58.0	58.0	1229-1243 bp	0.8	73.2
psbZ-trnG-GCC_p3	GTGTACCCGTCGTATTTGCT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	906-920 bp	0.8	73.2
psbZ-trnG-GCC_p4	ACCCGTCGTATTTGCTTCTC	CGACGTTGTTTTTCGACTGG	50.0	50.0	58.0	58.0	1225-1239 bp	0.8	73.1
psbZ-trnG-GCC_p5	TTTTTCTGGCTCGGCTATCC	CGACGTTGTTTTTCGACTGG	50.0	50.0	58.0	58.0	1836-1850 bp	0.8	72.6