Hotspot

rpoB-trnC-GCA

LSC · 28482-29727 · 1246 bp

Back to Berchemia

Pi 0.0785

MarkerSeek score 84.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 239
pi: 0.0785
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GATACCGGGGCTTTGCAATA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1610-1719 bp	1.0	91.1
rpoB-trnC-GCA_p2	CCGGGGCTTTGCAATATTTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1606-1715 bp	1.0	90.9
rpoB-trnC-GCA_p3	GATACCGGGGCTTTGCAATA	CCCTCTGGCTCTCCAATCTA	50.0	55.0	58.0	57.9	1978-1999 bp	0.8	72.2
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1815-1835 bp	0.8	72.1
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	CCCTCTGGCTCTCCAATCTA	50.0	55.0	58.0	57.9	2094-2115 bp	0.8	72.1