Hotspot

trnS-GCU-trnG-UCC

LSC · 8734-9963 · 1230 bp

Back to Berchemia

Pi 0.0982
MarkerSeek score 83.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1230
variable_sites
275
pi
0.0982
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 849-1299 bp 1.0 63.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 848-1298 bp 1.0 63.7
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 851-1301 bp 1.0 62.6
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 850-1300 bp 1.0 62.6
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 850-1300 bp 1.0 61.8