Hotspot

rps16-trnQ-UUG

LSC · 6603-7351 · 749 bp

Back to Berchemia

Pi 0.132

MarkerSeek score 85.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 237
pi: 0.132
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGATGTCGAGTCAAGAGC	CTCAGGTAATTTGGCGACGA	50.0	50.0	58.0	58.0	1405-1422 bp	0.8	73.4
rps16-trnQ-UUG_p2	CTCGAGCTCCATCATGTACG	CTCAGGTAATTTGGCGACGA	55.0	50.0	58.0	58.0	1478-1491 bp	0.8	73.4
rps16-trnQ-UUG_p3	AACGATGTCGAGTCAAGAGC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1492-1509 bp	0.8	72.9
rps16-trnQ-UUG_p4	AACGATGTCGAGTCAAGAGC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1497-1514 bp	0.8	72.9
rps16-trnQ-UUG_p5	AACGGATCGTGTCCTTCAAG	CTCAGGTAATTTGGCGACGA	50.0	50.0	57.9	58.0	1329-1346 bp	0.8	72.9