Hotspot

trnK-UUU-rps16

LSC · 4490-5458 · 969 bp

Back to Berchemia

Pi 0.122

MarkerSeek score 84.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 276
pi: 0.122
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1113-1149 bp	0.8	75.7
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1112-1148 bp	0.8	75.7
trnK-UUU-rps16_p3	ATTGGTACCACAGTAGGGCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	1316-1352 bp	0.8	75.4
trnK-UUU-rps16_p4	ATTGGTACCACAGTAGGGCT	CGTTGCAATTGATGTTCGCT	50.0	45.0	58.0	58.0	1467-1503 bp	0.8	74.8
trnK-UUU-rps16_p5	ATTGGTACCACAGTAGGGCT	TCGTTGCAATTGATGTTCGC	50.0	45.0	58.0	58.0	1468-1504 bp	0.8	74.8