Hotspot

matK-trnK-UUU

LSC · 3711-4452 · 742 bp

Back to Berchemia

Pi 0.0798

MarkerSeek score 84.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 145
pi: 0.0798
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGCCGTGATCATGAGCAAAT	GTCGTCTACAGCCTTTAGCC	45.0	55.0	58.0	58.1	1499-1526 bp	0.8	73.6
matK-trnK-UUU_p2	AGCCGTGATCATGAGCAAAT	AGTGCCAATCCAACACAAGT	45.0	45.0	58.0	57.9	1431-1447 bp	0.8	73.2
matK-trnK-UUU_p3	ACTCGTGAAGAAAAAGCCGT	GTCGTCTACAGCCTTTAGCC	45.0	55.0	58.1	58.1	1931-1958 bp	0.8	73.2
matK-trnK-UUU_p4	ACTCGTGAAGAAAAAGCCGT	AGTGCCAATCCAACACAAGT	45.0	45.0	58.1	57.9	1863-1879 bp	0.8	72.8
matK-trnK-UUU_p5	AGCCGTGATCATGAGCAAAT	AATGTTTTGATTGCGCTGGG	45.0	45.0	58.0	57.9	988-991 bp	0.8	71.9