Hotspot

ndhD

SSC · 116703-118205 · 1503 bp

Back to Berberis

Pi 0.00427

MarkerSeek score 36.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 25
pi: 0.00427
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTCCCTTTGGCGTGATTCAA	GATACCCTGGGACGGATGTA	45.0	55.0	58.0	58.0	2459-2490 bp	1.0	82.2
ndhD_p2	TTTCCCTTTGGCGTGATTCA	GATACCCTGGGACGGATGTA	45.0	55.0	58.0	58.0	2460-2491 bp	1.0	82.2
ndhD_p3	TTCCCTTTGGCGTGATTCAA	TTATGGCATGAGACAACCCG	45.0	50.0	58.0	58.0	2338-2369 bp	1.0	82.0
ndhD_p4	TTTCCCTTTGGCGTGATTCA	TTATGGCATGAGACAACCCG	45.0	50.0	58.0	58.0	2339-2370 bp	1.0	82.0
ndhD_p5	TTCCCTTTGGCGTGATTCAA	AAATGATACCCTGGGACGGA	45.0	50.0	58.0	58.1	2463-2494 bp	1.0	81.3