Hotspot

trnI-CAU-ycf2

IRb · 87437-87721 · 285 bp

Back to Berberis

Pi 0.0271

MarkerSeek score 70.40

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 285
variable_sites: 32
pi: 0.0271
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	ATAGACGTCGTTTTGCCCTG	50.0	50.0	58.0	58.3	340-517 bp	1.0	88.4
trnI-CAU-ycf2_p2	AGTTGGGCGCTTTAACCATT	ATAGACGTCGTTTTGCCCTG	45.0	50.0	58.4	58.3	341-518 bp	1.0	85.5
trnI-CAU-ycf2_p3	TTGGGCGCTTTAACCATTCA	ATAGACGTCGTTTTGCCCTG	45.0	50.0	58.4	58.3	339-516 bp	1.0	85.5
trnI-CAU-ycf2_p4	CCAATTATGAGTTGGGCGCT	ATAGACGTCGTTTTGCCCTG	50.0	50.0	58.6	58.3	350-527 bp	1.0	83.5
trnI-CAU-ycf2_p5	GAGTTGGGCGCTTTAACCAT	ATAGACGTCGTTTTGCCCTG	50.0	50.0	58.8	58.3	342-519 bp	1.0	81.8