Hotspot

rps12-clpP

LSC · 70656-71176 · 521 bp

Back to Berberis

Pi 0.0213

MarkerSeek score 65.80

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 37
pi: 0.0213
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	ATTGGCTGTCTTGTGTTTCT	ACTTTTATGGACGCAAGCGA	40.0	45.0	55.5	58.2	628-644 bp	1.0	65.9
rps12-clpP_p2	ATTGGCTGTCTTGTGTTTCT	GCCGACCTGGAAAGAGATAC	40.0	55.0	55.5	57.8	646-662 bp	1.0	65.5
rps12-clpP_p3	TGGCTGTCTTGTGTTTCTAA	ACTTTTATGGACGCAAGCGA	40.0	45.0	54.8	58.2	626-642 bp	1.0	60.8
rps12-clpP_p4	TTGGCTGTCTTGTGTTTCTA	ACTTTTATGGACGCAAGCGA	40.0	45.0	54.8	58.2	627-643 bp	1.0	60.8
rps12-clpP_p5	TGGCTGTCTTGTGTTTCTAA	GCCGACCTGGAAAGAGATAC	40.0	55.0	54.8	57.8	644-660 bp	1.0	60.3