Hotspot

ndhC-trnV-UAC

LSC · 51584-53159 · 1576 bp

Back to Berberis

Pi 0.00901
MarkerSeek score 67.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1576
variable_sites
52
pi
0.00901
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCGGTCCTTCACTAATTGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1671-1726 bp 1.0 84.2
ndhC-trnV-UAC_p2 CGGTCCTTCACTAATTGGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1670-1725 bp 1.0 84.2
ndhC-trnV-UAC_p3 CCTTCACTAATTGGGGCCAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1666-1721 bp 1.0 82.7
ndhC-trnV-UAC_p4 GGTCCTTCACTAATTGGGGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 1669-1724 bp 1.0 82.6
ndhC-trnV-UAC_p5 GTCCTTCACTAATTGGGGCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 1668-1723 bp 1.0 82.6