Hotspot

trnF-GAA-ndhJ

LSC · 49181-49900 · 720 bp

Back to Berberis

Pi 0.00522

MarkerSeek score 59.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 14
pi: 0.00522
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTTGATGCCCGAAAGTTGGA	50.0	45.0	58.1	58.0	808-847 bp	1.0	84.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GATAATCATCCGCGCCTGAA	50.0	50.0	58.1	58.2	834-873 bp	1.0	83.0
trnF-GAA-ndhJ_p3	TATGGAAATGATGCACCGCA	TTTGATGCCCGAAAGTTGGA	45.0	45.0	58.0	58.0	907-915 bp	0.411765	56.5
trnF-GAA-ndhJ_p4	TATGGAAATGATGCACCGCA	GATAATCATCCGCGCCTGAA	45.0	50.0	58.0	58.2	933-941 bp	0.411765	55.0
trnF-GAA-ndhJ_p5	TATGGAAATGATGCACCGCA	TTGATGCCCGAAAGTTGGAT	45.0	45.0	58.0	57.8	906-914 bp	0.411765	54.9