Hotspot

rps4-trnT-UGU

LSC · 46864-47262 · 399 bp

Back to Berberis

Pi 0.00666

MarkerSeek score 56.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 9
pi: 0.00666
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	509-534 bp	1.0	82.1
rps4-trnT-UGU_p2	GTAAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	525-550 bp	1.0	81.6
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	477-502 bp	1.0	81.5
rps4-trnT-UGU_p4	GTAAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.7	57.8	493-518 bp	1.0	81.0
rps4-trnT-UGU_p5	GCGATTGGTTTCGAGGATCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	569-594 bp	1.0	80.9