Hotspot

petN-psbM

LSC · 28730-29378 · 649 bp

Back to Berberis

Pi 0.0189

MarkerSeek score 71.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 32
pi: 0.0189
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTCT	45.0	38.1	58.0	55.9	798-808 bp	1.0	60.4
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCATTGTTCATTCT	50.0	38.1	57.8	55.9	804-814 bp	1.0	58.8
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCATTGTTCATTCT	50.0	38.1	57.6	55.9	744-754 bp	1.0	58.0
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTC	45.0	40.0	58.0	54.3	798-808 bp	1.0	55.7
petN-psbM_p5	TTGCTTGGGCTGCTTTAATG	TGCTACTGCATTGTTCATTCT	45.0	38.1	57.3	55.9	796-806 bp	1.0	54.5