primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTAACCCA	TGGGCTGATATCATCAACCG	20	20	50.0	50.0	57.8	57.5	0.0	5.7	0.763440	78	539	508-635	1.000000	80.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTAACCCAC	TGGGCTGATATCATCAACCG	20	20	50.0	50.0	57.8	57.5	0.0	5.7	0.765006	78	539	507-634	1.000000	80.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACAATCCACTGCCTTAACCC	TGGGCTGATATCATCAACCG	20	20	50.0	50.0	57.8	57.5	0.0	5.7	0.765006	78	539	509-636	1.000000	80.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTAACCCA	GGGCTGATATCATCAACCGT	20	20	50.0	50.0	57.8	57.4	0.0	5.7	0.765206	78	539	507-634	1.000000	80.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTGCCTTAACCCAC	GGGCTGATATCATCAACCGT	20	20	50.0	50.0	57.8	57.4	0.0	5.7	0.766772	78	539	506-633	1.000000	80.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTACCAACGGTATGGACGA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	57.9	58.0	10.6	7.6	0.151611	4390	4964	716-755	1.000000	85.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4390	4964	651-690	1.000000	85.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4390	4964	650-689	1.000000	85.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	0.0	7.6	0.290178	4390	4964	665-704	1.000000	84.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4390	4964	660-699	1.000000	84.3
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTCT	20	21	45.0	38.1	58.0	55.9	0.0	0.0	3.137832	28730	29378	798-808	1.000000	60.4
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCATTGTTCATTCT	20	21	50.0	38.1	57.8	55.9	0.0	0.0	3.322916	28730	29378	804-814	1.000000	58.8
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCATTGTTCATTCT	20	21	50.0	38.1	57.6	55.9	0.0	0.0	3.482651	28730	29378	744-754	1.000000	58.0
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTC	20	20	45.0	40.0	58.0	54.3	0.0	0.0	3.717181	28730	29378	798-808	1.000000	55.7
petN-psbM_p5	petN-psbM	5	TTGCTTGGGCTGCTTTAATG	TGCTACTGCATTGTTCATTCT	20	21	45.0	38.1	57.3	55.9	0.0	0.0	3.879185	28730	29378	796-806	1.000000	54.5
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.3	57.9	14.7	0.0	0.398577	46864	47262	509-534	1.000000	82.1
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GTAAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.7	57.9	0.0	0.0	0.439081	46864	47262	525-550	1.000000	81.6
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CTCCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.3	57.8	14.7	0.0	0.506331	46864	47262	477-502	1.000000	81.5
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GTAAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.546835	46864	47262	493-518	1.000000	81.0
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GCGATTGGTTTCGAGGATCA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.480753	46864	47262	569-594	1.000000	80.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TTTGATGCCCGAAAGTTGGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.063602	49181	49900	808-847	1.000000	84.7
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GATAATCATCCGCGCCTGAA	20	20	50.0	50.0	58.1	58.2	0.0	12.0	0.256128	49181	49900	834-873	1.000000	83.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	TATGGAAATGATGCACCGCA	TTTGATGCCCGAAAGTTGGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.061595	49181	49900	907-915	0.411765	56.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	TATGGAAATGATGCACCGCA	GATAATCATCCGCGCCTGAA	20	20	45.0	50.0	58.0	58.2	0.0	12.0	0.254121	49181	49900	933-941	0.411765	55.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TATGGAAATGATGCACCGCA	TTGATGCCCGAAAGTTGGAT	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.264264	49181	49900	906-914	0.411765	54.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCGGTCCTTCACTAATTGGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.235670	51584	53159	1671-1726	1.000000	84.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGTCCTTCACTAATTGGGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.235670	51584	53159	1670-1725	1.000000	84.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCTTCACTAATTGGGGCCAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	4.4	0.0	0.427413	51584	53159	1666-1721	1.000000	82.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGTCCTTCACTAATTGGGGC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.433315	51584	53159	1669-1724	1.000000	82.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GTCCTTCACTAATTGGGGCC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.433315	51584	53159	1668-1723	1.000000	82.6
rbcL-psaI_p1	rbcL-psaI	1	ATTTGAATTTGAAGCAATGGA	AGAGGGAAAGTTGAAAATTGTCA	21	23	28.6	34.8	52.5	56.5	0.0	0.0	10.948302	58360	61282	2652-2978	0.882353	45.2
rbcL-psaI_p2	rbcL-psaI	2	TTTGAATTTGAAGCAATGGAT	AGAGGGAAAGTTGAAAATTGTCA	21	23	28.6	34.8	52.5	56.5	0.0	0.0	10.948302	58360	61282	2651-2977	0.882353	45.2
rbcL-psaI_p3	rbcL-psaI	3	TCTGGAAGGAAATCAAATTTGA	AGAGGGAAAGTTGAAAATTGTCA	22	23	31.8	34.8	53.9	56.5	22.5	0.0	10.572477	58360	61282	2668-2994	0.882353	45.1
rbcL-psaI_p4	rbcL-psaI	4	TCTGGAAGGAAATCAAATTTGAA	AGAGGGAAAGTTGAAAATTGTCA	23	23	30.4	34.8	54.7	56.5	23.9	0.0	10.763415	58360	61282	2668-2994	0.882353	45.1
rbcL-psaI_p5	rbcL-psaI	5	TCTGGAAGGAAATCAAATTTGA	AAGAGGGAAAGTTGAAAATTGTCA	22	24	31.8	33.3	53.9	57.2	22.5	0.0	10.910583	58360	61282	2669-2995	0.882353	45.1
rps12-clpP_p1	rps12-clpP	1	ATTGGCTGTCTTGTGTTTCT	ACTTTTATGGACGCAAGCGA	20	20	40.0	45.0	55.5	58.2	0.0	22.3	2.732897	70656	71176	628-644	1.000000	65.9
rps12-clpP_p2	rps12-clpP	2	ATTGGCTGTCTTGTGTTTCT	GCCGACCTGGAAAGAGATAC	20	20	40.0	55.0	55.5	57.8	0.0	0.0	2.762833	70656	71176	646-662	1.000000	65.5
rps12-clpP_p3	rps12-clpP	3	TGGCTGTCTTGTGTTTCTAA	ACTTTTATGGACGCAAGCGA	20	20	40.0	45.0	54.8	58.2	0.0	22.3	3.375270	70656	71176	626-642	1.000000	60.8
rps12-clpP_p4	rps12-clpP	4	TTGGCTGTCTTGTGTTTCTA	ACTTTTATGGACGCAAGCGA	20	20	40.0	45.0	54.8	58.2	0.0	22.3	3.375270	70656	71176	627-643	1.000000	60.8
rps12-clpP_p5	rps12-clpP	5	TGGCTGTCTTGTGTTTCTAA	GCCGACCTGGAAAGAGATAC	20	20	40.0	55.0	54.8	57.8	0.0	0.0	3.405206	70656	71176	644-660	1.000000	60.3
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	ATAGACGTCGTTTTGCCCTG	20	20	50.0	50.0	58.0	58.3	7.6	10.5	0.287189	87437	87721	340-517	1.000000	88.4
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	AGTTGGGCGCTTTAACCATT	ATAGACGTCGTTTTGCCCTG	20	20	45.0	50.0	58.4	58.3	7.6	10.5	0.650125	87437	87721	341-518	1.000000	85.5
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	TTGGGCGCTTTAACCATTCA	ATAGACGTCGTTTTGCCCTG	20	20	45.0	50.0	58.4	58.3	7.6	10.5	0.655996	87437	87721	339-516	1.000000	85.5
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	CCAATTATGAGTTGGGCGCT	ATAGACGTCGTTTTGCCCTG	20	20	50.0	50.0	58.6	58.3	7.6	10.5	0.885399	87437	87721	350-527	1.000000	83.5
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GAGTTGGGCGCTTTAACCAT	ATAGACGTCGTTTTGCCCTG	20	20	50.0	50.0	58.8	58.3	7.6	10.5	1.104076	87437	87721	342-519	1.000000	81.8
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	AAATATATAGGGCGCGGACG	20	20	50.0	50.0	58.2	57.7	15.0	8.8	0.445593	108653	111372	2232-2900	0.941176	83.3
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AGGATTCGAACCTACGACCA	GCGCGGACGAATTTCTTTTT	20	20	50.0	45.0	58.2	58.3	15.0	8.8	0.474807	108653	111372	2221-2889	0.941176	83.1
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGAACGGGTTACCTACTCCT	GCGCGGACGAATTTCTTTTT	20	20	55.0	45.0	58.1	58.3	0.0	8.8	0.460211	108653	111372	2323-2991	0.941176	83.0
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CGGGCCCTTTCATTCTGAAT	AAATATATAGGGCGCGGACG	20	20	50.0	50.0	58.2	57.7	22.4	8.8	0.521202	108653	111372	2303-2971	0.941176	82.5
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGAACGGGTTACCTACTCCT	AAATATATAGGGCGCGGACG	20	20	55.0	50.0	58.1	57.7	0.0	8.8	0.430996	108653	111372	2334-2983	0.882353	80.8
ndhF-rpl32_p1	ndhF-rpl32	1	TGGAAGTGGAACCCAAGGTA	CGTTTCTTTGGAACTGCC	20	18	50.0	50.0	58.2	54.3	0.0	0.0	5.837899	113608	114634	995-1104	1.000000	47.6
ndhF-rpl32_p2	ndhF-rpl32	2	GCTGTAGGGACAACGAGAAG	CGTTTCTTTGGAACTGCC	20	18	55.0	50.0	58.0	54.3	0.0	0.0	5.662655	113608	114634	1039-1148	1.000000	47.5
ndhF-rpl32_p3	ndhF-rpl32	3	CGACGAAGACTTTTTGCTGC	CGTTTCTTTGGAACTGCC	20	18	50.0	50.0	58.3	54.3	0.0	0.0	5.996830	113608	114634	1057-1166	1.000000	47.5
ndhF-rpl32_p4	ndhF-rpl32	4	TTTTGCTGCTGTAGGGACAA	CGTTTCTTTGGAACTGCC	20	18	45.0	50.0	57.6	54.3	0.0	0.0	6.016989	113608	114634	1046-1155	1.000000	47.4
ndhF-rpl32_p5	ndhF-rpl32	5	TTTTTGCTGCTGTAGGGACA	CGTTTCTTTGGAACTGCC	20	18	45.0	50.0	57.6	54.3	0.0	0.0	6.016989	113608	114634	1047-1156	1.000000	47.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTTGCGCGACAAACAAGTAA	AGCGTGTCTACCGATTTCAC	20	20	40.0	50.0	57.2	58.0	19.8	0.0	0.834809	114794	115321	543-603	1.000000	81.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTGCGCGACAAACAAGTAA	CAGCGTGTCTACCGATTTCA	20	20	40.0	50.0	57.2	58.0	19.8	0.0	0.836180	114794	115321	544-604	1.000000	81.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTTGCGCGACAAACAAGTAA	TCCTAAGAGCAGCGTGTCTA	20	20	40.0	50.0	57.2	57.9	19.8	0.0	0.951256	114794	115321	553-613	1.000000	80.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTTGCGCGACAAACAAGTAA	CCTAAGAGCAGCGTGTCTAC	20	20	40.0	55.0	57.2	57.8	19.8	0.0	1.023274	114794	115321	552-612	1.000000	79.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTTGCGCGACAAACAAGTAA	CTAAGAGCAGCGTGTCTACC	20	20	40.0	55.0	57.2	57.8	19.8	0.0	1.023274	114794	115321	551-611	1.000000	79.4
ccsA-ndhD_p1	ccsA-ndhD	1	TGCAATTGTAGCTTCTATGGGA	TGCACTATCAGTTGACAAGGT	22	21	40.9	42.9	57.5	57.2	7.4	0.0	4.271844	116463	116702	403-434	1.000000	53.8
ccsA-ndhD_p2	ccsA-ndhD	2	TGCAATTGTAGCTTCTATGGGA	TCCGGATTTTGTTTTTGCACT	22	21	40.9	38.1	57.5	57.2	7.4	0.0	4.312416	116463	116702	418-449	1.000000	53.2
ccsA-ndhD_p3	ccsA-ndhD	3	TGCAATTGTAGCTTCTATGGGA	CGATCTGTATCCTTCTACCCG	22	21	40.9	52.4	57.5	57.2	7.4	0.0	4.335651	116463	116702	458-489	1.000000	52.4
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAATTGTAGCTTCTATGGGA	TTGCACTATCAGTTGACAAGGT	22	22	40.9	40.9	57.5	57.9	7.4	0.0	4.587463	116463	116702	404-435	1.000000	51.3
ccsA-ndhD_p5	ccsA-ndhD	5	CTGCAATTGTAGCTTCTATGGG	TGCACTATCAGTTGACAAGGT	22	21	45.5	42.9	57.2	57.2	10.7	0.0	4.595284	116463	116702	404-435	1.000000	51.2
ndhD_p1	ndhD	1	TTCCCTTTGGCGTGATTCAA	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	58.0	58.0	0.0	22.2	0.017077	116703	118205	2459-2490	1.000000	82.2
ndhD_p2	ndhD	2	TTTCCCTTTGGCGTGATTCA	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	58.0	58.0	0.0	22.2	0.017077	116703	118205	2460-2491	1.000000	82.2
ndhD_p3	ndhD	3	TTCCCTTTGGCGTGATTCAA	TTATGGCATGAGACAACCCG	20	20	45.0	50.0	58.0	58.0	0.0	11.0	0.048664	116703	118205	2338-2369	1.000000	82.0
ndhD_p4	ndhD	4	TTTCCCTTTGGCGTGATTCA	TTATGGCATGAGACAACCCG	20	20	45.0	50.0	58.0	58.0	0.0	11.0	0.048664	116703	118205	2339-2370	1.000000	82.0
ndhD_p5	ndhD	5	TTCCCTTTGGCGTGATTCAA	AAATGATACCCTGGGACGGA	20	20	45.0	50.0	58.0	58.1	0.0	22.2	0.123909	116703	118205	2463-2494	1.000000	81.3
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	ACGTATTTTGATTTGCGGGA	TTGCTTGACGAGTTCGAAGT	20	20	40.0	45.0	56.0	57.8	0.0	4.9	2.180640	128758	128877	173-251	1.000000	74.6
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	ACGTATTTTGATTTGCGGGA	CGAAGTGGAAGAACGACTGA	20	20	40.0	50.0	56.0	57.6	0.0	0.0	2.379026	128758	128877	159-237	1.000000	73.0
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	ACGTATTTTGATTTGCGGGA	CTTGACGAGTTCGAAGTGGA	20	20	40.0	50.0	56.0	57.6	0.0	20.2	2.379026	128758	128877	170-248	1.000000	73.0
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	ACGTATTTTGATTTGCGGGA	TCGAAGTGGAAGAACGACTG	20	20	40.0	50.0	56.0	57.6	0.0	0.0	2.379026	128758	128877	160-238	1.000000	73.0
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	ACGTATTTTGATTTGCGGGA	TGACGAGTTCGAAGTGGAAG	20	20	40.0	50.0	56.0	57.6	0.0	20.2	2.379026	128758	128877	168-246	1.000000	73.0
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	ATAGACGTCGTTTTGCCCTG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.3	58.0	10.5	7.6	0.287189	152515	152799	340-517	1.000000	84.2
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	ATAGACGTCGTTTTGCCCTG	AGTTGGGCGCTTTAACCATT	20	20	50.0	45.0	58.3	58.4	10.5	7.6	0.650125	152515	152799	341-518	1.000000	81.3
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	ATAGACGTCGTTTTGCCCTG	TTGGGCGCTTTAACCATTCA	20	20	50.0	45.0	58.3	58.4	10.5	7.6	0.655996	152515	152799	339-516	1.000000	81.3
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	ATAGACGTCGTTTTGCCCTG	CCAATTATGAGTTGGGCGCT	20	20	50.0	50.0	58.3	58.6	10.5	7.6	0.885399	152515	152799	350-527	1.000000	79.4
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	ATAGACGTCGTTTTGCCCTG	GAGTTGGGCGCTTTAACCAT	20	20	50.0	50.0	58.3	58.8	10.5	7.6	1.104076	152515	152799	342-519	1.000000	77.7