Hotspot

trnE-UUC-rpoB

LSC · 12001-12846 · 846 bp

Back to Berardia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
846
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGATTTGAACCCTCGTTGT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 990 bp 1.0 61.5
trnE-UUC-rpoB_p2 TTTGAACCCTCGTTGTCTCC AGTTCTTCTGTCAAACCCTGA 50.0 42.9 57.8 56.8 986 bp 1.0 60.3
trnE-UUC-rpoB_p3 GGGATTTGAACCCTCGTTGT TCAAACCCTGATCAATGAACCT 50.0 40.9 58.1 57.6 980 bp 1.0 60.1
trnE-UUC-rpoB_p4 GGGATTTGAACCCTCGTTGT TCTTCTGTCAAACCCTGATCA 50.0 42.9 58.1 56.6 987 bp 1.0 59.8
trnE-UUC-rpoB_p5 GGGATTTGAACCCTCGTTGT GTCAAACCCTGATCAATGAACC 50.0 45.5 58.1 57.6 981 bp 1.0 59.7