Hotspot

psbM-trnD-GUC

LSC · 10964-11603 · 640 bp

Back to Berardia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	TCTGATTCCCCTGGGATTGT	45.0	50.0	58.3	58.0	770 bp	1.0	77.8
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	TCTGATTCCCCTGGGATTGT	45.0	50.0	57.6	58.0	769 bp	1.0	76.9
psbM-trnD-GUC_p3	AAATGAACAGCGCAGTAGCA	TCTGATTCCCCTGGGATTGT	45.0	50.0	58.5	58.0	772 bp	1.0	76.1
psbM-trnD-GUC_p4	AATGAACAGCGCAGTAGCAA	TCTGATTCCCCTGGGATTGT	45.0	50.0	58.5	58.0	771 bp	1.0	76.1
psbM-trnD-GUC_p5	ATGAACAGCGCAGTAGCAAT	TTCAATAGGCAAGAGCACCG	45.0	50.0	58.3	58.3	748 bp	1.0	75.8