Hotspot

trnS-GCU-trnC-GCA

LSC · 8596-9352 · 757 bp

Back to Berardia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
757
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 908 bp 1.0 78.1
trnS-GCU-trnC-GCA_p2 ACGGAAAGAGAGGGATTCGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 890 bp 1.0 75.9
trnS-GCU-trnC-GCA_p3 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 844 bp 1.0 74.3
trnS-GCU-trnC-GCA_p4 AACGGAAAGAGAGGGATTCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.3 58.1 909 bp 1.0 73.9
trnS-GCU-trnC-GCA_p5 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 826 bp 1.0 72.1