Hotspot

trnM-CAU

LSC · 53437-53508 · 72 bp

Back to Bennettiodendron

Pi 0.0139
MarkerSeek score 60.50
Primer available yes
Label trnM-CAU

Feature Metadata

label_name
trnM-CAU
feature_type
tRNA
region
LSC
length_bp
72
variable_sites
1
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU_p1 TGTGACGGGTTCCATTTCTT AGATACCATGGATTCGGATATCT 45.0 39.1 57.4 56.2 145 bp 1.0 40.6
trnM-CAU_p2 CGATGTGACGGGTTCCATTT AGATACCATGGATTCGGATATCT 50.0 39.1 58.6 56.2 148 bp 1.0 40.5
trnM-CAU_p3 TAATCCATCGATGTGACGGG AGATACCATGGATTCGGATATCT 50.0 39.1 57.2 56.2 156 bp 1.0 40.5
trnM-CAU_p4 CGATGTGACGGGTTCCATTT TTAGATACCATGGATTCGGA 50.0 40.0 58.6 52.9 150 bp 1.0 40.5
trnM-CAU_p5 ATGTGACGGGTTCCATTTCT AGATACCATGGATTCGGATATCT 45.0 39.1 57.1 56.2 146 bp 1.0 40.5